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complex N-linked glycan biosynthesis (vertebrates)

State Icon Reaction EC-Number Confidence maximal confidence score from EnzymeDetector
UDP-galactose + {beta-D-GlcNAc-(1rarr2)-[beta-D-GlcNAc-(1rarr4)]-alpha-D-Man-(1rarr3)-[beta-D-GlcNAc-(1rarr2)-[beta-D-GlcNAc-(1rarr6)]-alpha-D-Man-(1rarr6)]-beta-D-Man-(1rarr4)-beta-D-GlcNAc-(1rarr4)-beta-D-GlcNAc}-N-Asn-[protein] <=> {beta-D-Gal-(1rarr4)-beta-D-GlcNAc-(1rarr2)-[beta-D-Gal-(1rarr4)-beta-D-GlcNAc-(1rarr4)]-alpha-D-Man-(1rarr3)-[beta-D-Gal-(1rarr4)-beta-D-GlcNAc-(1rarr2)-[beta-D-Gal-(1rarr4)-beta-D-GlcNAc-(1rarr6)]-alpha-D-Man-(1rarr6)]-beta-D-Man-(1rarr4)-beta-D-GlcNAc-(1rarr4)-beta-D-GlcNAc}-N-Asn-[protein] + UDP + H+ 2.4.1.38 beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase

UDP-N-acetyl-D-glucosamine + Man3GlcNAc4-[protein] <=> {beta-D-GlcNAc-(1rarr2)-[beta-D-GlcNAc-(1rarr4)]-alpha-D-Man-(1rarr3)-[beta-D-GlcNAc-(1rarr2)-alpha-D-Man-(1rarr6)]-beta-D-Man-(1rarr4)-beta-D-GlcNAc-(1rarr4)-beta-D-GlcNAc}-N-Asn-[protein] + UDP + H+ 2.4.1.145 alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

CMP-N-acetylneuraminate + {beta-D-Gal-(1rarr4)-beta-D-GlcNAc-(1rarr2)-[beta-D-Gal-(1rarr4)-beta-D-GlcNAc-(1rarr4)]-alpha-D-Man-(1rarr3)-[beta-D-Gal-(1rarr4)-beta-D-GlcNAc-(1rarr2)-[beta-D-Gal-(1rarr4)-beta-D-GlcNAc-(1rarr6)]-alpha-D-Man-(1rarr6)]-beta-D-Man-(1rarr4)-beta-D-GlcNAc-(1rarr4)-beta-D-GlcNAc}-N-Asn-[protein] <=> {beta-D-Gal-(1rarr4)-beta-D-GlcNAc-(1rarr2)-[beta-D-Gal-(1rarr4)-beta-D-GlcNAc-(1rarr4)]-alpha-D-Man-(1rarr3)-[alpha-D-Sial-(2rarr3)-beta-D-Gal-(1rarr4)-beta-D-GlcNAc-(1rarr2)-[beta-D-Gal-(1rarr4)-beta-D-GlcNAc-(1rarr6)]-alpha-D-Man-(1rarr6)]-beta-D-Man-(1rarr4)-beta-D-GlcNAc-(1rarr4)-beta-D-GlcNAc}-N-Asn-[protein] + CMP + H+ 2.4.99.6 N-acetyllactosaminide alpha-2,3-sialyltransferase

UDP-N-acetyl-D-glucosamine + Man3GlcNAc4-[protein] <=> {beta-D-GlcNAc-(1rarr2)-alpha-D-Man-(1rarr3)-[beta-D-GlcNAc-(1rarr2)-alpha-D-Man-(1rarr6)]-[beta-D-GlcNAc-(1rarr4)]-beta-D-Man-(1rarr4)-beta-D-GlcNAc-(1rarr4)-beta-D-GlcNAc}-N-Asn-[protein] + UDP + H+ 2.4.1.144 beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

UDP-N-acetyl-D-glucosamine + Man5GlcNAc2-[protein]_(isomer_5A1,2) <=> H+ + Man5GlcNAc3-[protein] + UDP 2.4.1.101 alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

CMP-N-acetylneuraminate + {beta-D-Gal-(1rarr4)-beta-D-GlcNAc-(1rarr2)-[beta-D-Gal-(1rarr4)-beta-D-GlcNAc-(1rarr4)]-alpha-D-Man-(1rarr3)-[alpha-D-Sial-(2rarr3)-beta-D-Gal-(1rarr4)-beta-D-GlcNAc-(1rarr2)-[beta-D-Gal-(1rarr4)-beta-D-GlcNAc-(1rarr6)]-alpha-D-Man-(1rarr6)]-beta-D-Man-(1rarr4)-beta-D-GlcNAc-(1rarr4)-beta-D-GlcNAc}-N-Asn-[protein] <=> {alpha-D-Sial-(2rarr6)-beta-D-Gal-(1rarr4)-beta-D-GlcNAc-(1rarr2)-[beta-D-Gal-(1rarr4)-beta-D-GlcNAc-(1rarr4)]-alpha-D-Man-(1rarr3)-[alpha-D-Sial-(2rarr3)-beta-D-Gal-(1rarr4)-beta-D-GlcNAc-(1rarr2)-[beta-D-Gal-(1rarr4)-beta-D-GlcNAc-(1rarr6)]-alpha-D-Man-(1rarr6)]-beta-D-Man-(1rarr4)-beta-D-GlcNAc-(1rarr4)-beta-D-GlcNAc}-N-Asn-[protein] + CMP + H+ 2.4.99.1 beta-galactoside alpha-(2,6)-sialyltransferase

GDP-L-fucose + Man3GlcNAc4-[protein] <=> N4-{beta-D-GlcNAc-(1rarr2)-alpha-D-Man-(1rarr3)-[beta-D-GlcNAc-(1rarr2)-alpha-D-Man-(1rarr6)]-beta-D-Man-(1rarr4)-beta-D-GlcNAc-(1rarr4)-[alpha-L-Fuc-(1rarr6)]-beta-D-GlcNAc}-N-Asn-[protein] + GDP + H+ 2.4.1.68 glycoprotein 6-alpha-L-fucosyltransferase

UDP-N-acetyl-D-glucosamine + {beta-D-GlcNAc-(1rarr2)-[beta-D-GlcNAc-(1rarr4)]-alpha-D-Man-(1rarr3)-[beta-D-GlcNAc-(1rarr2)-alpha-D-Man-(1rarr6)]-beta-D-Man-(1rarr4)-beta-D-GlcNAc-(1rarr4)-beta-D-GlcNAc}-N-Asn-[protein] <=> {beta-D-GlcNAc-(1rarr2)-[beta-D-GlcNAc-(1rarr4)]-alpha-D-Man-(1rarr3)-[beta-D-GlcNAc-(1rarr2)-[beta-D-GlcNAc-(1rarr6)]-alpha-D-Man-(1rarr6)]-beta-D-Man-(1rarr4)-beta-D-GlcNAc-(1rarr4)-beta-D-GlcNAc}-N-Asn-[protein] + H+ + UDP 2.4.1.155 alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase

UDP-N-acetyl-D-glucosamine + Man3GlcNAc3-[protein] <=> Man3GlcNAc4-[protein] + UDP + H+ 2.4.1.143 alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

Man5GlcNAc3-[protein] + H2O <=> Man3GlcNAc3-[protein] + D-mannose 3.2.1.114 mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase

Visualization of the pathway